KASP Filter for Exome Capture SNP
Interval selection for KASP marker design from WheatCAP exome capture data (https://triticeaetoolbox.org/wheat/). Designed markers are plotted above a density map comparing SNP from all observed lines shared by or different between the two selected parents. Markers are colored by the genomne specificity of the surrounding sequence, with darker markers being designed from less specific sequences, and are plotted by chromosome position and relative score. Markers are optimized for the temperature settings recommended for KASP PCR. Developed by Noah DeWitt, NC State University, Crop and Soil Sciences.
general protocol for KASP